MammaTyper

Report Generator

For in-vitro diagnostic use

Language:

Pathologist: Department: Date of measurement: Plate ID: MammaTyper® Lot:

Instructions:
Step 1, Select instrument
Step 2, Check the data format
Step 3, Upload the data
Select both files for FAM and HEX/VIC/JOE channels and upload them together to the software.

Note:
- Make sure the sample names are consistent among all the wells within the same row. Failure to follow the naming rule may result in errors during the calculation;
- Regardless how many samples (1-8) were run on the plate, make sure you export the data for the whole plate except for position D10-D12 and E10-E12. Any missing wells on the file will result in errors during the calculation.

V2.0

ERBB2	0/Ultra Low	Low 1+/2+(ISH -ve)	Pos 2+(ISH +ve)/ 3+
40-ΔΔCq	<38.2≥ <40.4≥
ESR1	Negative < 1%	Low < 10%	Positive ≥ 10%
40-ΔΔCq	<36.9≥ <38.0≥
PGR	Negative < 1%	Low < 20%	Positive ≥ 20%
40-ΔΔCq	<35.1≥ <36.5≥
MKI67	5%	20%	30%	60%
40-ΔΔCq	34.7	35.9	36.7	37.4

ERBB2	0/Ultra Low	Low 1+/2+(ISH -ve)	Pos 2+(ISH +ve)/ 3+
40-ΔΔCq	<38.1≥ <40.0≥
ESR1	Negative < 1%	Low < 10%	Positive ≥ 10%
40-ΔΔCq	<36.7≥ <37.7≥
PGR	Negative < 1%	Low < 20%	Positive ≥ 20%
40-ΔΔCq	<35.2≥ <36.6≥
MKI67	5%	20%	30%	60%
40-ΔΔCq	34.8	35.8	36.4	37.0

ERBB2	0/Ultra Low	Low 1+/2+(ISH -ve)	Pos 2+(ISH +ve)/ 3+
40-ΔΔCq	<38.3≥ <40.4≥
ESR1	Negative < 1%	Low < 10%	Positive ≥ 10%
40-ΔΔCq	<37.1≥ <38.2≥
PGR	Negative < 1%	Low < 20%	Positive ≥ 20%
40-ΔΔCq	<35.0≥ <36.3≥
MKI67	5%	20%	30%	60%
40-ΔΔCq	35.1	36.3	37.0	37.7

ERBB2	0/Ultra Low	Low 1+/2+(ISH -ve)	Pos 2+(ISH +ve)/ 3+
40-ΔΔCq	<38.4≥ <40.4≥
ESR1	Negative < 1%	Low < 10%	Positive ≥ 10%
40-ΔΔCq	<37.5≥ <38.5≥
PGR	Negative < 1%	Low < 20%	Positive ≥ 20%
40-ΔΔCq	<34.9≥ <36.2≥
MKI67	5%	20%	30%	60%
40-ΔΔCq	34.6	35.9	36.7	37.4

ERBB2	0/Ultra Low	Low 1+/2+(ISH -ve)	Pos 2+(ISH +ve)/ 3+
40-ΔΔCq	<38.3≥ <40.4≥
ESR1	Negative < 1%	Low < 10%	Positive ≥ 10%
40-ΔΔCq	<36.9≥ <38.0≥
PGR	Negative < 1%	Low < 20%	Positive ≥ 20%
40-ΔΔCq	<34.8≥ <36.2≥
MKI67	5%	20%	30%	60%
40-ΔΔCq	34.2	35.5	36.3	37.0